Description:
Serine protease that exhibits strong proteolytic activity on a wide variety of denatured and native proteins of high molecular weight. Because of its rapid proteolytic inactivation of endogenous nucleases, it can be used for isolation of mRNA and high molecular weight DNA. Preferentially cleaves bonds next to the carbonyl group of N-substituted hydrophobic, aliphatic, and aromatic amino acids. Inhibited by DFP, Hg2+, and PMSF. Not inactivated by metal chelators, sulfhydryl reagents, TLCK, or TPCK.
Catalogue Number: 539480
Brand Family: Calbiochem
Product Information:
CAS number 39450-01-6
Activity ≥30 mAnsonU/mg dry weight
Unit of Definition One mAnson unit is defined as the amount of enzyme that will liberate 1.0 µmol of Folin-positive amino acids (calculated as tyrosine) per min at 25°C, pH 7.5.
EC number 3.4.21.14
Form White lyophilized solid
Formulation Lyophilized from calcium acetate
Molar Mass 28500
Biological Information:
Specific Activity ≥40 mAnsonU/mg protein
Physicochemical Information
Contaminants DNase: no detectable nicking activity with pBR322, incubation for 6 h at 37°C; RNase: no ribonuclease activity with MS2 RNA detected after incubation for 6 h at 37°C
pH value optimum 7.5 - 12.0
Solubility 50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0
Safety Information:
R Phrase R: 36/37/38-42
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation.
S Phrase S: 22-24-26-36/37
Do not breathe dust.
Avoid contact with skin.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing and gloves.
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special Instructions Following reconstitution, store in the refrigerator (4°C). Stock solutions prepared in 50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0 are stable for up to 1 year at 4°C.
