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Lympho Spin Medium / PBMC Spin Medium

品牌：pluriSelect Life Science

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储存：RT，避光

Details

PBMC Spin is a sterile, ready-to-use density medium for the isolation of peripheral blood mononuclear cells from fresh human blood or buffy coat with a high yield. Cells can be enriched by a single-step density gradient centrifugation with PBMC Spin Medium. Through combination with Leuko Spin Medium in a double gradient centridugation it is possible to isolate and separate granulocytes and PBMCs in one step with high yield and purity. PBMC Spin Medium can be used as substitute for Ficoll-Paque¹, Pancoll² and Lymphoprep³ without any need to change the existing protocols. The enriched cells can be used for a wide range of downstream applications such as MACS⁴, pluriBead or stem cell research.

¹ Ficoll-Paque is a trademark of GE Healthcare/Cytiva
² Pancoll is a trademark of PAN-Biotech GmbH
³ Lymphoprep is a trademark of Alere Technologies Inc
⁴ MACS is a trademark of Miltenyi Biotec GmbH

Application

Separation scheme for PBMC enrichment with PBMC Spin Medium

At first carefully layer the sample material on top of the PBMC Spin Medium. Avoid a mixing of the two phases. Alternatively it’s possible to use pluriMate tubes. The barrier prevents the mixing of the two phases and enables to pour off the enriched cells after the first centrifugation step. After the density centrifugation aspirate the upper layer (Plasma and dilution buffer). Afterwards transfer the mononuclear cell layer to a new conical tube and wash the cells.